Conventional small RNA sequencing workflows are optimized for short, minimally structured molecules. As a result, highly structured and modification-rich RNAs, like tRNAs and their functional fragments, are often underrepresented or inaccurately quantified. MSR-seq™ reduces these biases to deliver a more complete and informative profile of the small non-coding RNA landscape.
Simultaneously measure RNA abundance, modification signatures, aminoacylation (charging), and fragmentation patterns from a single NGS assay. Together, these readouts provide a more complete picture of small RNA behavior from each sample.
Abundance
tRNA, miRNA, snoRNA, snRNA, mtRNA, rRNA, Y RNA, vtRNA and more.
Epitranscriptomic Modifications
m1A, m66A, m2A, ms2i6A, I, m1I, m1G, m22G, m7G, Q, W, m3C, s2U, s4U, m3U
Charging
tRNA Aminoacylation (Charging) Ratios
Fragmentation
5' RNA fragments
Separate ligation steps before and after reverse transcription reduces bias associated with modification induced RT stops.
A proprietary hairpin adapter architecture supports broad capture of small RNAs with available 3' ends while minimizing adapter-dimer formation and ligation bias.
Solid-state support enables a column-free workflow that helps reduce purification bias, simplify lab handling, and lower input requirements to 1 microgram of total RNA.
Barcode-enabled multiplexing supports parallel sample processing, improving consistency while reducing hands-on time and handling-related variability.